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MacWorld: Complete Mac Interactive
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Macworld Complete Mac Interactive CD)(1994).iso
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NIH Image 1.55 f (non fpu)
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Macros
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Gel Plotting Macros
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1994-02-01
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219 lines
var {Global variables}
RoiLeft,RoiTop,RoiWidth,RoiHeight:integer;
GelPid,MarkedPid,nLanes,count,ppv:integer;
SaveRoiLeft,SaveRoiTop,hOffset,vOffset:integer;
MinY,MaxY,xscale,yscale:real;
VerticalLanes,Uncalibrated:boolean;
procedure MarkLane;
var
left,top,width,height:integer;
GelNotCalibrated:boolean;
min,max:real;
begin
GetRoi(left,top,width,height);
if width=0 then begin
PutMessage('Please outline the next lane.');
exit;
end;
if (VerticalLanes and (left=RoiLeft))
or (not VerticalLanes and (top=RoiTop)) then begin
PutMessage('This lane has already been marked.');
exit;
end;
nLanes:=nLanes+1;
ChoosePic(GelPid);
if VerticalLanes then begin
MakeRoi(left+hOffset,RoiTop+vOffset,RoiWidth,RoiHeight);
RoiLeft:=left;
rUser1[nLanes]:=left;
end else begin
MakeRoi(RoiLeft+hOffset,top+vOffset,RoiWidth,RoiHeight);
RoiTop:=top;
rUser1[nLanes]:=top;
end;
GetPlotData(count,ppv,min,max);
if min<MinY then MinY:=min;
if max>MaxY then MaxY:=max;
SelectPic(MarkedPid);
if VerticalLanes
then MakeRoi(left,RoiTop,RoiWidth,RoiHeight)
else MakeRoi(RoiLeft,top,RoiWidth,RoiHeight);
DrawBoundary;
SetFontSize(9);
if VerticalLanes then begin
SetText('Centered, No Background');
MoveTo(RoiLeft+RoiWidth/2,RoiTop-6);
end else begin
SetText('Right Justified, No Background');
MoveTo(RoiLeft-2,RoiTop+RoiHeight/2);
end;
Write(nLanes:1);
RestoreRoi;
SelectPic(MarkedPid);
end;
macro 'Mark First Lane [1]';
var
GelWidth,GelHeight,left,width:integer;
margin,leftm,rightm,topm,bottomm:integer;
begin
RequiresVersion(1.54);
if WindowTitle='Temp' then begin
PutMessage('To restart, select the first lane in the gel.');
exit;
end;
GetRoi(RoiLeft,RoiTop,RoiWidth,RoiHeight);
if RoiWidth=0 then begin
PutMessage('The Setup macro expects the Gel to be displayed and the first lane outlined.');
exit;
end;
VerticalLanes:=RoiHeight>RoiWidth;
Uncalibrated:=not Calibrated;
SaveState;
GelPid:=PidNumber;
SetForegroundColor(255);
SetBackgroundColor(0);
MinY:=999999;
MaxY:=-999999;
nLanes:=0;
margin:=20;
GetPicSize(GelWidth,GelHeight);
leftm:=RoiLeft;
if leftm>margin then leftm:=margin;
rightm:=GelWidth-(RoiWidth+RoiLeft);
if rightm>margin then rightm:=margin;
bottomm:=GelHeight-(RoiHeight+RoiTop);
if bottomm>margin then bottomm:=margin;
topm:=RoiTop;
if topm>margin then topm:=margin;
if VerticalLanes
then MakeRoi(RoiLeft-leftm,RoiTop-topm,
GelWidth-RoiLeft+leftm,RoiHeight+topm+bottomm)
else MakeRoi(RoiLeft-leftm,RoiTop-topm,
RoiWidth+leftm+rightm,GelHeight-RoiTop+topm);
Duplicate('Temp');
MarkedPid:=PidNumber;
SaveRoiLeft:=RoiLeft;
SaveRoiTop:=RoiTop;
hOffset:=RoiLeft-leftm;
vOffset:=RoiTop-topm;
RoiLeft:=leftm;
RoiTop:=topm;
SelectPic(GelPid);
KillRoi;
SelectPic(MarkedPid);
MakeRoi(RoiLeft,RoiTop,RoiWidth,RoiHeight);
if VerticalLanes
then RoiLeft:=-1
else RoiTop:=-1;
SetPlotScale(0,0);
MarkLane;
end;
macro 'Mark Next Lane [2]';
var
left,top,width,height:integer;
GelNotCalibrated:boolean;
min,max:real;
begin
if nLanes=0 then begin
PutMessage('Please use the "Mark First Lane" macro first.');
Exit;
end;
MarkLane;
end;
macro 'Plot Lanes [3]';
var
left,top,width,height:integer;
GelNotCalibrated:boolean;
i,LaneLength:integer;
xmin,xmax,ymin,ymax,min,max:real;
MaxPlotWindowHeight,NeededSize:integer;
PlotTop,PlotBottom,PlotWidth,PlotHeight:integer;
hMargin,vMargin,SavePlotTop,PlotWindowSize:integer;
PlotWinHeight,PlotWinWidth,PlotsPid:integer;
begin
if nLanes=0 then begin
PutMessage('Please use the Setup and Mark macros first.');
Exit;
end;
if VerticalLanes
then LaneLength:=RoiHeight
else LaneLength:=RoiWidth;
PlotWidth:=LaneLength;
if PlotWidth<500 then PlotWidth:=500;
if PlotWidth>(2*LaneLength) then PlotWidth:=2*LaneLength;
PlotHeight:=PlotWidth/2;
if PlotHeight<200 then PlotHeight:=200;
if PlotHeight>400 then PlotHeight:=400;
MaxPlotWindowHeight:=800;
hMargin:=5;
vMargin:=5;
PlotWinHeight:=nLanes*PlotHeight+2*vMargin;
if PlotWinHeight>MaxPlotWindowHeight then begin
PlotWinHeight:=MaxPlotWindowHeight;
PlotHeight:=(PlotWinHeight-2*vMargin)/nLanes;
end;
PlotWinWidth:=PlotWidth+2*hMargin;
PlotWindowSize:=PlotWinWidth*PlotWinHeight;
if PlotWindowSize>UndoBufferSize then begin
NeededSize:=(PlotWindowSize+0.05*PlotWindowSize) div 1024;
PutMessage('Use Preferences(Options Menu) to increase the Undo buffer size to at least ',NeededSize:1,'K.');
KillRoi;
exit;
end;
SetForegroundColor(255);
SetBackgroundColor(0);
SetNewSize(PlotWinWidth,PlotWinHeight);
MakeNewWindow('Plots');
PlotsPid:=PidNumber;
if UnCalibrated then begin
SetText('Left Justified');
SetFontSize(12);
MoveTo(6,vmargin+1);
Write('uncalibrated');
end;
xmin:=0;
xmax:=count-1;
ymin:=MinY;
ymax:=MaxY;
xscale:=PlotWidth/(xmax-xmin);
yscale:=PlotHeight/(ymax-ymin);
PlotTop:=vMargin;
SavePlotTop:=PlotTop;
RoiLeft:=SaveRoiLeft;
RoiTop:=SaveRoiTop;
SetLineWidth(1);
for i:=1 to nLanes do begin
PlotBottom:=PlotTop+(ymax-ymin)*yscale;
ChoosePic(GelPid);
if VerticalLanes
then MakeRoi(rUser1[i]+hOffset,RoiTop,RoiWidth,RoiHeight)
else MakeRoi(RoiLeft,rUser1[i]+vOffset,RoiWidth,RoiHeight);
GetPlotData(count,ppv,min,max);
SelectPic(PlotsPid);
MoveTo(hMargin,PlotBottom);
LineTo(PlotWinWidth-hMargin,PlotBottom);
MoveTo(hMargin,PlotBottom-(PlotData[0]-ymin)*yscale);
for i:=1 to count-1 do
LineTo(hMargin+i*xscale,PlotBottom-(PlotData[i]-ymin)*yscale);
PlotTop:=PlotTop+PlotHeight;
end;
nLanes:=0;
ResetCounter;
SetOptions('Area');
WandAutoMeasure(true);
AdjustAreas(true);
LabelParticles(false);
IncludeInteriorHoles(true);
SetFontSize(9);
SetText('Centered');
if Uncalibrated
then SetScale(0,'pixels')
else SetScale(sqrt(xscale*yscale/ppv),'unit');
RestoreState;
end;